flagship biosciences

Fibrous capsule measurement for coated devices

Analysis of fibrous capsules is a common procedure in evaluation of coated devices.  Microvessel analysis was tested for use in identifying the fibrous capsule differences between a novel coating and a control polypropylene in a subcutaneous rodent implant model.

Two immunofluorescent images comparing novel coating with

Confirmation of a novel coating onto polypropylene mesh. Anti-fibronectin immunofluorescent staining of uncoated control (left) and surface treated (right).

 

 

Histopath images showing control polypropylene versus surface treated

Control polypropylene (left) versus surface treated (right)

Microvessel algorithm was modified to detect the fibrous capsule, as shown below.

Image analysis results of use of angiogenesis algorithm in fibrous capsule detection

Representative markup image of fibrous capsule surrounding polypropylene fibers

In this example, as in almost all image analysis problems, the goal is to get from images to data. Below is some example output, there clearly is a measurable difference in fibrous capsule area between the new biosurface and the control.  More samples would be required to access statistical significance.quantitative differences in the new biosurface material

  1. Rob Kellar Reply

    Biocompatibility of materials is evaluated using a variety of methods. One parameter that provides a rather useful indication of the biocompatibility of a material is the extent of the fibrous capsule that is formed by the host tissue in response to the implanted material. Most all materials stimulate the formation of a fibrous capsule to some extent. If the capsule formation is robust, the function of the biomaterial may be compromised in its end use or application. Therefore, we routinely use quantitative data on fibrous capsule measurements to screen or provide an indication of the “biocompatibility” of various biomaterials, devices, or even surface coatings. Using the whole slide digital scanning technology we are able to generate data from the entire specimen rather than only selected fields of view.

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