A scaffold-based, three-dimensional, human dermal fibroblast culture (3DFC) was used as a cardiac patcha to stimulate revascularization and preserve left ventricular (LV) function of the infarcted LV in severe combined immunodeficient (SCID) mice.
Scanning Electron Micrograph of Anginera (Vicryl scaffold with cultured human fibroblasts). Scale bar = 50 mm
Representative images at time of explantation (14 days) after injection of TTC. 3DFC-treated heart (14 days) demonstrating new vasculature.
Gs-1 lectin–reacted sections from 30-day 3DFC treatment group. Scale bar = 100 mm.
The histopathology image above illustrates the differences between the venules, arterioles, and capillaries. Image analysis is able to identify these vessels and segregate them based on size.
aKellar, RS, et al. (2001) Scaffold-based three-dimensional human fibroblast culture provides a structural matrix that supports angiogenesis in infarcted heart tissue. Circulation. 104(17):2063-8.
