The eye serves as an important organ for toxicology research. Below is a rodent H&E ocular cross-section, followed by pathologist-trained pattern recognition of each layer. This provides a larger and faster region of interest drawing than would be used with manual ROI drawing by a technician or pathologist. Depending on the performance, Flagship will use pattern recognition in some cases and manual technician drawing with pathologist supervision as shown below.
In the example above, there is some error in the automated pattern recognition approach for estabilishing regions of interest. The Flagship pathologist assesses the performance on each slide, and will determine if the accuracy is acceptable, or if manual region of interest drawing is required as shown below.
Once Flagship identifies the cell layers, either manually or with histology pattern recognition (or a mixture of both), cell and area measurements can be combined to give quantitative changes of cell layer densities or other layer abnormalities between control and treated animals. After either manual ROI or histology pattern recognition, cell counting and cell density can be determined on the inner and outer nuclear layers, or the ganglion cell layer, for example.