White fat measurement is shown below with a Flagship algorithm that first segments and then individually identifies each fat vacuole. Statistics of area and perimeter per vacuole are counted, and histograms can be output as required. Some results are shown below. In areas where vacuoles are either not easily seen by eye, or where they are very small and merged together, it is best to eliminate these by restricting the vacuole size to be larger.
Orginal image of white fat on the left, with the image analysis results on the right
Area is graphed on the left, on a per vacuole basis. On the right is perimeter. Both can be useful, depending on the application.
2nd example region
In cases like lower right area of this image, the vacuole area threshold can be increased to eliminate problematic areas
Elongated vacuoles can be eliminated by increasing the “circularity” threshold
